Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: Comparison of the expression of inflammatory mediators (A) IL-1β, (B) IL-6 and (C) IL-8, as well as (D) STAT3 between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (E) IL-1β, (F) IL-6, (G) IL-8 and (H) STAT3 mRNA levels and ST3Gal1 expression. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. Data in A-D are presented as box and whisker plots, and those in E-H as scatter plots. Statistical significance for group comparisons in A-D was assessed using the unpaired t-test; correlation analyses in E-H were performed via Pearson's correlation coefficient. **P<0.01.

Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

Techniques: Comparison, Expressing, Gene Expression, Whisker Assay

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: Comparison of (A) MUC2, (B) TFF3 and (C) CDX2 mRNA levels between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (D) MUC2, (E) TFF3 and (F) CDX2 mRNA levels and ST3Gal1 expression. Data in A-C are presented as box and whisker plots, and those in D-F as scatter plots. Statistical significance for group comparisons in A-C was assessed using unpaired t-test. Correlation analyses in D-F were performed using Pearson's correlation coefficient. *P<0.05 and **P<0.01. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. *P<0.05 and **P<0.01. NS, not significant; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Comparison, Expressing, Whisker Assay, Gene Expression